The standard 10X Genetics workflow was used. An asymetrical sequencing profile is used to sequence stand alone 10X single cell 5 libraries. Each chip can process 8 samples at once, capturing 80,000 in less than 20 minutes. Single Cell Multiome ATAC v2 + 3' Gene Expression v3. Cells were then delivered immediately to the MGH NextGen Sequencing Core Facility and core staff prepared cDNA libraries using the 10X Genetics v3.0 kits. Immunology Neurology Stem Cell Biology Immuno-oncology Infectious disease Single cell libraries prepared using the 10X Chromium Controller are Illumina compatable and will be sequenced using the NovaSeq 6000. The 10X Chromium Controller generates thousands of single cell nano-reactions to barcode up to 10,000 cells per lane for high throughput single cell analysis. Sample #2 - #8 : Human or Mouse / T cell or B cell Sample #1 : Human or Mouse / T cell or B cell Single Cell 5' Expression Library v2 + V(D)J Immune Enrichment Single Cell 5' Expression v2 V(D)J Library The sample #1 cost is applicable for each 10X Chromium Chip required. The average affiliate pricing per sample is $2050.63 for Single Cell 3' expression, $2050.63 for Single Cell 5' expression, $2065.63 for Single Cell V(D)J immune profiling, $2135.63 for Single Cell 5' Expression + V(D)J immune profiling, $1975.63 for ATAC and $3350.63 for Single Cell Multiome ATAC + 3' Gene Expression when performing an 8 sample project. The tiered cost structure for 10X Chromium Controller library preparation is structured to obtain cost recovery on the single use 8 sample 10X Chromium Chip. Targeted cell/nuclei counts and reads per cell/nuclei are project specific and will determine the sequencing required and the associated cost. In one application, the instrument uses synthetic gel beads to partition thousands of separate enzymatic reactions necessary for downstream DNA sequencing. The MGH NextGen Sequencing Core Core Services PRICING Overview Basic pricing for NextGen library construction and sequencing services is given below. using the 10X Genomics Chromium Controller Instrument (10X Genomics. provides two unique key technologies to the UF research community. 1Klarman Cell Observatory, Broad Institute of MIT and Harvard, Cambridge, MA 02142. Analyze full length paired B-cell or T-cell receptors, surface protein expression, antigen specificity, and gene expression, all from a single cell.Ĭhromium Single Cell ATAC (Assay for Transposase Accessible Chromatin) allows you to analyze chromatin accessibility at the single cell level, providing insights into cell types and states, and deeper understanding of gene regulatory mechanisms.The pricing for 10X Chromium Controller library preparations does not include sequencing cost on the NovaSeq 6000. The Chromium instrument developed by California-based 10X Genomics inc. Explore cellular heterogeneity, identify rare cell types, novel targets, biomarkers, cell types and states.Ĭhromium Single Cell Immune Profiling provides a multiomic solution to your immunology questions. Please contact information about technical procedures, library prep and more and Rita Holdhus at the Genomics Core facility for sequencing.Ĭhromium Single Cell Gene Expression provides single cell transcriptome 3’ gene expression profiles from hundreds to tens of thousands of cells. From assessing tumor heterogeneity and stem cell composition, to dissecting neuronal populations-the technological advancements provided by the Chromium Single Cell Gene Expression Solution, along with turnkey software tools, allow you to maximize insight from any sample type 1.įor more information about the technology, please visit 10X Genomics and the broshure The Power of Single Cell PartitioningĪ checklist of the materials required can be found at the bottom of the page. Go beyond traditional gene expression analysis to characterize cell populations, cell types, cell states, and more on a cell-by-cell basis. Our latest improvements vastly increase sensitivity, so you can detect even more unique transcripts per cell 1. The Chromium Single Cell Gene Expression Solution provides an unparalleled approach to uncover cell-to-cell gene expression variability and identify rare cell types from complex biological samples. Until recently, most molecular studies have relied on bulk analysis, combining all cells into a single average readout. The power of dissecting cell-type differences in complex biological systems is critical to our understanding of cellular contributions during development and in disease progression. The Chromium X Series is compatible with all of the company’s existing low throughput and standard single cell assays and is supported by the 10x Genomics Cloud Analysis offering for data.
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